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Not every PCOS patient has the same metabolic picture and carnitine is not appropriate for every presentation

For transfection, siRNAs were delivered to a final concentration of 10 nM using Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific) as detailed by the manufacturer

Cultured mammalian cells might offer a better option for producing properly folded and functional mammalian proteins with appropriate post-translational modifications (Geisse et al