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Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)
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Determining the correct B12 dosage can be tricky, as the appropriate amount depends on factors such as age, the severity of deficiency, and any coexisting conditions

While it was originally developed with therapeutic uses in mind, it remains primarily a research tool for studying fat metabolism and weight-management mechanisms

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